Equipments Details
Description
The Genome Editing Shared Resource at Rutgers Cancer Institute of New Jersey was established in 2016. GESR is now a University core facility led by the Office of Research Advancement (ORAd) under Rutgers' Office of Research and Economic Development. It also functions as an NCI-designated cancer center resource with joint scientific and administrative/fiscal oversight with Rutgers Cancer Institute of New Jersey.
The GESR serves as one of America’s largest, most comprehensive university-based research centers. The GESR's main facility is located in New Brunswick with a satellite site in Newark. Its main focus is to provide expertise and technical services required to manipulate the genomes of animals utilizing CRISPR/Cas9-mediated gene editing and, eventually, genome editing of various cell lines including human cells. Standard services such as mouse embryonic stem cell manipulation, in vitro fertilization, embryo or sperm cryopreservation, mouse rederivation, genotyping and breeding services are also available.
The GESR offers the following services :
CRISPR/CAS9 -Mediated Gene Editing in Mouse
- gene knock-out
- gene replacement (amino acid changes)
- epitope tagging, including fluorescent protein tagging
conditional models
DNA Microinjection for transgenic generation
-Transgene DNA Purification available as an additional service
BAC DNA Microinjection for BAC transgenesis
ES Cell Line Injection
- ES Cell preparation and Karyotyping available as additional services
CRISPR Target Validation (for Gene Editing in Mouse)
Mouse Cryopreservation:
- Sperm Cryopreservation
- Embryo Cryopreservation
In Vitro Fertilization
- Frozen/Fresh sperm for line import, recovery or rapid expansion
Mouse Line Rederivation
- Live Mice as Source
- Frozen Embryos as Source
Genotyping
- The GESR offers genotyping of lines generated as an additional service
- The GESR will design and validate genotyping assays as needed
Fees for GESR services are available upon request, please email gecf-ru@rutgers.edu
The GESR serves as one of America’s largest, most comprehensive university-based research centers. The GESR's main facility is located in New Brunswick with a satellite site in Newark. Its main focus is to provide expertise and technical services required to manipulate the genomes of animals utilizing CRISPR/Cas9-mediated gene editing and, eventually, genome editing of various cell lines including human cells. Standard services such as mouse embryonic stem cell manipulation, in vitro fertilization, embryo or sperm cryopreservation, mouse rederivation, genotyping and breeding services are also available.
The GESR offers the following services :
CRISPR/CAS9 -Mediated Gene Editing in Mouse
- gene knock-out
- gene replacement (amino acid changes)
- epitope tagging, including fluorescent protein tagging
conditional models
DNA Microinjection for transgenic generation
-Transgene DNA Purification available as an additional service
BAC DNA Microinjection for BAC transgenesis
ES Cell Line Injection
- ES Cell preparation and Karyotyping available as additional services
CRISPR Target Validation (for Gene Editing in Mouse)
Mouse Cryopreservation:
- Sperm Cryopreservation
- Embryo Cryopreservation
In Vitro Fertilization
- Frozen/Fresh sperm for line import, recovery or rapid expansion
Mouse Line Rederivation
- Live Mice as Source
- Frozen Embryos as Source
Genotyping
- The GESR offers genotyping of lines generated as an additional service
- The GESR will design and validate genotyping assays as needed
Fees for GESR services are available upon request, please email gecf-ru@rutgers.edu