Mass Spectrometry Laboratory

    Roman Brukh (Manager)

Equipment/facility: Facility

  • Location

    73 Warren St., Newark, NJ 07102

    United States

Description

High resolution mass measurements, which can provide elemental composition for analytes.

Electrospray ionization (ESI), Atmospheric Pressure Chemical ionization (APCI), and Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry are used for wide range of applications by research groups and students.

Fragmentation methods include in-source collision activated dissociation (IS-CAD), collision cell activated dissociation (C-CAD), sustained off-resonance irradiation collision activated dissociation (SORI-CAD), infrared multi-photon dissociation (IRMPD), and electron capture dissociation (ECD).

The mass spectrometry facility has three mass spectrometers available:
1. Bruker FTMS equipped with a 7.0 T magnet, and an Apollo II Dual ESI/MALDI source that can provide simultaneous operation in both modes.

The HPLC system is available to be coupled with ESI/APCI source for separation and analysis of complex mixtures.

MS/MS or MS/MS/MS fragmentation data provides a tremendous amount of information for analysis of unknown samples.

2. Bruker UltrafleXtreme MALDI-TOF/TOF is used for a variety of MALDI applications, including protein identification, peptide fingerprinting, polymer research, and structure identification for a wide spectrum synthetic and biological compounds.

3. Agilent 6890 GC equipped with 5973 MSD GC-MS system provides routine mass spectrometry analysis for our graduate and undergraduate students.

High Performance Liquid Chromatography is coupled with ESI or APCI sources to allow for the separation of mixtures and subsequent analysis.

Sample applications are compound elucidation, drug impurity analysis, natural product characterization, high-end proteomics studies, polymer and metabolomics researc

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Electrospray ionization
Mass spectrometry
Ionization
Desorption
Ion sources
Atmospheric pressure
Lasers
Polymers
Students
High performance liquid chromatography
Mass spectrometers
Biological Products
Complex Mixtures
Magnets
Photons
Irradiation
Impurities
Infrared radiation
Peptides
Electrons