ACTIN IN EMBRYOGENESIS--MECHANISMS OF REGULATION

Project Details

Description

The ability of cells to properly change shape, migrate and
divide, within the embryo, can determine the success or failure
of embryonic development. Many of the motile events during
morphogenesis are directed by the cell's actin cytoskeleton. The
actin cytoskeleton of eukaryotic cells is a highly dynamic
assembly of assorted structures. Fortunately, there exists a
functional universality linking the actin regulatory proteins
from broadly diverse cell types. The specific goals of this
project are: a. Characterize the effects of sea urchin egg llOK, 95K, 50K,
and 13K (profilin?) actin binding proteins on actin filament
assembly and structure. In particular, the modulatory effects of
second messengers such as Ca , pH, phospholipid and the
nucleotide bound to the actin subunit will be analysed; b. Investigate the mechanism of Ca -sensitive regulation of egg
spectrin-actin crosslinking: c. Generate and characterize polyclonal and/or monoclonal
antibodies that monospecifically crossreact with the isolated and
characterized actin binding proteins; d. Employ the anti-actin binding protein antibodies for
immunocytochemical studies determining the spatio-temporal
association of the regulatory proteins with the various actin
cytoskeleton domains in oocytes, eggs and embryos; e. Examine the synthesis of the actin binding proteins during
oogenesis and embryogenesis using in situ labeling and
immunoprecipitation and/or autoradiography. Successful completion of the above aims will provide the
necessary correlations between the in vitro protein interactions
and the in vivo actin cytoskeletal localization and synthesis to
generate hypotheses describing the potential molecular mechanisms
modulating the actin cytoskeleton. Additionally, the production
of anti-actin binding protein antibodies will provide the
necessary probes to test the hypothetical molecules mechanisms in
vivo and to begin examining differential expression of the actin
binding proteins during embryogenesis.
StatusFinished
Effective start/end date12/31/893/31/95

Funding

  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development

ASJC

  • Medicine(all)
  • Immunology

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