AFFERENT REGULATION OF CHOLINERGIC FOREBRAIN NEURONS

Project Details

Description

The long-term goal will be to obtain a systematic and detailed
understanding of the organization and afferent regulation of forebrain
cholinergic projection neurons of the rat, especially in areas such as the
vertical and horizontal limb of the diagonal band and the sublenticular
substantia innominata. In order to achieve this goal, we will identify the
sources and chemical characteristics of the afferents the cholinergic
projection neurons using a series of combined immunocytochemical and tracer
techniques at both the light and electron microscope level. Specific
attempts will be made to study the fine synaptic articulation pattern of
these afferents on different parts of the cholinergic projection neuron
using the recently introduced section-Golgi technique.

We also propose to examine biochemically the effect of removal of
monoaminergic afferents on the activity of choline acetyltransferase in
cholinergic projection neurons. As part of these experiments, we will
determine the age-related susceptibility of cholinergic projection neurons
to afferent deprivation. Quantitative light and electron microscopic
studies of local and extrinsic synapses will also proceed in order to
characterize the synaptic input to the cholinergic projection neurons in
quantitative terms (synapse/cell ration, cell and synaptic densities).

This study will provide important information about the organization of the
cholinergic projection system and will test the hypothesis that dysfunction
or loss of specific afferents might result in atrophy or degeneration of
basal forebrain cholinergic neurons. The integrity of these neurons is
apparently necessary for normal cognitive function and atrophy or
degeneration of basal forebrain cholinergic neurons is reliably observed in
Alzheimer's disease.
StatusActive
Effective start/end date12/31/893/31/25

Funding

  • National Institute of Neurological Disorders and Stroke: $421,139.00
  • National Institute of Neurological Disorders and Stroke: $331,388.00
  • National Institute of Neurological Disorders and Stroke: $349,155.00
  • National Institute of Neurological Disorders and Stroke: $413,389.00
  • National Institute of Neurological Disorders and Stroke: $349,217.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $92,519.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $426,481.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $468,357.00
  • National Institute of Neurological Disorders and Stroke: $357,457.00
  • National Institute of Neurological Disorders and Stroke: $352,749.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $339,788.00
  • National Institute of Neurological Disorders and Stroke: $326,605.00
  • National Institute of Neurological Disorders and Stroke: $270,493.00
  • National Institute of Neurological Disorders and Stroke: $320,652.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $326,020.00
  • National Institute of Neurological Disorders and Stroke: $542,914.00
  • National Institute of Neurological Disorders and Stroke: $262,615.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke: $473,007.00
  • National Institute of Neurological Disorders and Stroke: $30,000.00
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke

ASJC

  • Medicine(all)
  • Neuroscience(all)
  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Anatomy

Fingerprint

Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.