AUTOIMMUNE ROLE IN AIDS DEMENTIA COMPLEX

Project Details

Description

Infection with the human immunodeficiency virus (HIV) commonly
involves the central nervous system. The most frequent
neuropsychiatric syndrome due to infection with HIV is a
debilitating progressive demintia complex (ADC). The
pathogenesis of ADC is not known, but appears to be due to
undefined secondary viral effects rather than direct cell
infection. Based on our positive preliminary data-the
demonstration of specific antibrain antibody on Western blots and
indirect immunofluorescence- this proposal will explore the role
of autoantibodies (autoAbs) to brain components in ADC.
Specimens will be examined from existing banks of samples
collected from ADC patients and controls: sera (n greater than
1000), paired CSF and sera (n=100),and brain plus paired CSF and
sera (n=50). Ongoing collection of specimens will continue from
patients who are being evaluated by formal neuropsychological
and neurologic protocols. Extensive controls will be included.
CSF and serum will be examined for autoAb to whole brain,
specific brain antigens (including myelin basic protein,
gangliosides, n-CAM, galactocerebroside) and cell lystes
(including neuronal and glial lines). Both the technique of Western
immunoblots and indirect immunofluorescene will be used for the
above examination. Immunohistochemistry will be used to look
for in-situ brain autoAb. In addition, the immune complexes (IC)
we detect in the CSF from ADC patients will be isolated and
dissociated, and the IC components examined for brain reactivity
as well as HIV components. IC will be measured using 3 assays
(Raji cell, solid phase Clg, and PEG-ELISA) and isolated by Raji
cell elution or PEG precipitation. Data will be analyzed using
appropriate statistical methods. The proposal should help
determine whether an autoimmune process is linked to ADC. It
should also contribute more broadly to information on the
potential role of viruses and autoimmune disturbances in
dementing and neuropsychiatric diseases.
StatusFinished
Effective start/end date12/31/896/30/93

Funding

  • National Institute of Mental Health

ASJC

  • Medicine(all)
  • Clinical Neurology
  • Neurology
  • Infectious Diseases
  • Immunology

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