BIOCHEMICAL STUDIES ON HUMAN T CELL RECEPTOR MOLECULES

Project Details

Description

The main objective of this proposal is to study the human T cell antigen
receptor molecules through serological and biochemical means. One preliminary results on T cell antigen receptor, and those obtained by
others, suggest that this molecular should be polymorphic so as to perform
the recognition of different antigens by various T cell clones. We are
currently engaged in isolation of sufficient amounts of the T cell receptor
molecules from two T cell lines and cells of two patients with T cell
leukemia which would allow us to develop reagents directed at the constant
portion of these molecules. These reagents will be used for isolation and
biochemical characterization of T cell receptor molecules from various
sources. These reagents will also prove valuable in pursuing the questions
related to the mechanisms of expression of the genes coding for T cell
antigen receptor in different subpopulations of normal T cells and T cell
malignancies. More specifically, we will utilize obtained reagents for the
following purposes: 1. Generation of antibodies against constant region
of human T cell receptor molecules. 2. Initial biochemical
characterization of T cell receptor molecule isolated from various T cell
sources (MW, pI, presence of disulfide bonds). 3. Determination of cell
surface components closely associated with T cell receptor. 4. Study of
biosynthesis and processing of T cell receptor polypeptide chains isolated
from various T cell sources. 6. Characterization of carbohydrate
composition of T cell receptor polypeptide chains.
StatusFinished
Effective start/end date8/1/867/31/87

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

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