BIOCHEMISTRY OF GLYCOLIPIDS IN GASTRIC SECRETION

Project Details

Description

The specific goals of the proposed research are: to detemine the content
and composition of lipids in the undegraded mucus glycoprotein isolated
from gastric mucus; to establish the requirements of glyceroglucolipids for
for interacting with mucus glycoprotein, and to determine whether this
interaction involves the protein or carbohydrate component of the
glycoprotein; to provide the information as to the topography of lipids
within the undegraded gastric mucus glycoprotein polymer; to determine
whether glyceroglucolipids protect the mucus glycoprotein polymer from the
degradation by pepsin; to determine the effect of lipids on the rheological
properties of mucus glycoprotein, and J to evaluate the role of mucus
lipids in the process of H+ retardation by gastric mucus; to determine
whether activities of enzymes involved in the biosynthesis of
glyceroglucolipids in gastric mucosa of patients with gastric diseases
differ from that of normals. The undegraded mucus glycoproteins, isolated
from pig, dog and rat gastric mucus by the equilibrium centrifugation in
CsCl density gradient, will be used to study the content and composition of
lipids. Dissociation of the undegraded mucus glycoproteins into a central
protein component and the reduced glycoprotein monomers will be performed
by the reductive cleavage of dissulfide bridges. The effect of lipids on
the peptic digestion of mucus glycoprotein will be measured by the
trinitrophenylation method. Viscosity measurements and the study on the
role of lipids in the retardation of H+ diffusion by gastric mucus will be
performed on the intact and delipidated mucus and glycoprotein. Human
gastric mucosal biopsy specimens will be used to study the activities of
enzymes involved in the biosynthesis of glyceroglucolipids.
StatusFinished
Effective start/end date9/15/788/31/87

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)

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