BIOCHEMISTRY OF GLYCOLIPIDS IN GASTRIC SECRETION

Project Details

Description

The long term objective of this project is to explore the
biochemistry and function of glycolipids, phospholipids and neutral
lipids in gastrointestinal mucus in health and disease, and to
provide the information on the role of mucus lipids in gastric
mucosal protection. The specific goals set for the proposed
period are: to define the role of covalently bound fatty acids and
associated lipids in the viscoelastic, permselective and
hydrophobic behavior of mucus glycoproteins along the
gastrointestinal tract, and in the susceptibility of these
glycoproteins to various proteases; to ascertain the extent of
changes occurring in the covalently bound and associated lipids,
and in the physicochemical characteristics of gastric mucus
glycoprotein in stomach pathology; to assess the effect of
antiulcer drugs that enhance the mucosal defense and agents
affecting the integrity of mucus gel on the proteolytic
susceptibility and the hydrophobic, viscoelastic, and
permselective properties of gastric mucus and mucus
glycoprotein; to study the effect of antiulcer drugs that enhance
the mucosal defense on the acylation of mucus glycoprotein with
fatty acids by gastric mucosal cells in culture; to conduct the
measurements of the mucus glycoprotein fatty acyltransferase
enzyme activity in fundic and antral mucosal biopsies of patients
with gastric disease in order to provide an insight into the changes
taking place in gastric mucus gel in stomach pathology; and to
investigate the extent of changes occurring in mucus and mucosa
components of gastric mucosal barrier in disease by studying in
human gastric mucosal biopsies the activity of sulfotransferase
enzymes responsible for sulfation of structural and secretory
glycolipids.
StatusFinished
Effective start/end date9/15/788/31/93

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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