BIOCHEMISTRY OF SALIVARY LIPIDS

Project Details

Description

The long term objective of this project is to explore the biochemistry and
function of lipids in saliva and salivary concretions in health and
disease, and to define the relationship of these lipids to the protein and
glycoprotein components. The specific goals set for the proposed period
are: to establish the role of covalently bound and associated lipids in
the buoyant density, viscosity and hydrophobic behavior of salivary mucins
from caries-resistant (CR) and caries-susceptible (CS) subjects; to
evaluate the effect of covalently bound and associated lipids on the
susceptibility of salivary mucins to proteolytic degradation; to isolate
and characterize lipoproteins of parotid saliva from CR and CS subjects; to
determine the composition and content of lipids covalently bound and
associated with proline-rich glycoprotein of parotid saliva, and assess
differences with caries; to investigate the relationship of lipids to
proteins and glycoproteins in tooth enamel pellicle; to evaluate the role
of pellicle mucin and lipoprotein components in the retardation of
cariogenic acids diffusion and the contribution of lipids to this function
of pellicle; to characterize lipids of the mucous layer covering human oral
epithelium, explore their origin and relationship to other constituents,
and determine rheological and permselective properties of this mucous
layer; to investigate changes in lipids of the oral mucous layer in
subjects with aphthous stomatitis; to study substrate specificity,
subcellular location and kinetics of the enzyme responsible for the
acylation of salivary glycoproteins with fatty acids in rat salivary
glands; to conduct the measurements of the acyltransferase activity in
salivary gland biopsies of patients with salivary gland and systemic
disease; to measure the activities of enzymes responsible for sulfation of
secretory and structural glycolipids in salivary glands of patients with
salivary gland and systemic disease; and to pursue studies on lipids and
their relation to proteins and glycoproteins in the secretions of the major
and minor salivary glands of patients with salivary gland and systemic
diseases.
StatusFinished
Effective start/end date12/31/891/31/98

Funding

  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research

ASJC

  • Medicine(all)
  • Dentistry(all)
  • Biochemistry

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