CELL MEMBRANE SYNTHESIS IN THE CONTROL OF GROWTH

Project Details

Description

The assembly of the membrane of a lipid-containing
bacteriophage, phi 6, will be studied as a means of elucidating the
processes of membrane differentiation and translocation. Phi 6
contains a genome of three segments of double-stranded RNA
inside of a ployhedral procapsid, which is in turn inside of another
capsid shell. This double layered structure, called the
nucleocapsid, is enveloped by a membrane that contains
phospholipids and five species of protein that are specified by the
phage genome. The assembly of the lipid-containing membrane on
the particle is dependent upon the activity of protein P12, which
is the only phage specified protein that is not a part of the virion. The assembly process has been studied in our laboratory in great
detail using nonsense mutants of the phage. In addition, we have
made cDNA clones of the entire genome, and we have sequenced
the entire genome. We have also prepared expression vectors for
both E. coli and the Pseudomonad hosts of the phage and
demonstrated complementation activity of the cDNA material. We will investigate the assembly of the viral membrane by
studying the interaction between protein P12 and nascent viral
membrane proteins in vitro. Fusions of the membrane proteins
with alkaline phosphatase will be prepared as a means of
investigating whether particular parts of the proteins have
targeting sequences. Physiological studies will also be performed
to study the dependence of the localization of one or more of the
membrane proteins on the presence of protein P12 and several
other membrane proteins that may play important roles in the
formation of the membrane. The formation of membranes and the transfer of membrane
material is vital to many cellular processes such as organelle
formation, receptor mediated responses, virus formation, and
secretion of cellular materials. Understanding the processes
would be of use in combating viral diseases, in ameliorating
diseases due to dysfunction of hormone and metabolite receptor
responses, nerve impulse transmission, cellular differentiation and
proliferation.
StatusFinished
Effective start/end date12/31/896/30/05

Funding

  • National Institute of General Medical Sciences: $357,783.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $379,384.00
  • National Institute of General Medical Sciences: $361,253.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $368,424.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Virology
  • Cell Biology

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