Project Details

Description

Considerable evidence indicates an etiological role for
HTLV-III and related retroviruses in the current epidemic of
AIDS. The env gene-coded proteins of retroviruses play
important roles in the biological activities of these agents,
and are the major targets of the protective immune response
against these viruses. We have considerable experience in the
characterization of the structure, functional activities, and
immunological pro-erties of the env gene products of the murine
leukemia viruses, which represent an excellent retrovirus model
for understanding the recent human isolates such as HTLV-III.
We propose to perform similar characterizations of the AIDS
virus env components. Monoclonal antibodies will be prepared
against the purified glycoproteins of HTLV-III and LAV. The
localization and chemical nature of the epitopes on the env
proteins recognized by these antibodies antibodies will be
studied, and the neutralizing and cytotoxic titers of the
antibodies determined, as means of identifying those sites on
the env proteins which induce such activities. A binding
competition assay will be developed as an efficient method of
characterizing similar activities in sera of human subjects
exposed to the AIDS viruses. Using appropriate antisera, the
biosynthesis of the env gene products will be characterized,
the contribution of carbohydrates to the maturation and
function of these proteins will be determined, and the subunit
organization of the env components will be elucidated. The
effect of the purified HTLV-III env proteins on in vitro
hematopoietic cell differentiation and functional activities
will be determined. These studies should provide needed
insight into the nature of these molecules which should clarify
their functional roles in induction of AIDS, and which should
facilitate the design of an effective vaccine against these
viruses.
StatusFinished
Effective start/end date7/1/864/30/99

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)

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