CLONING OF TRANSFORMATION EFFECTOR AND SUPPRESSOR GENES

Project Details

Description

The proposed studies are designed to identify, molecularly clone
and characterize cellular genes which comprise and/or regulate
the biochemical pathways essential for the process of malignant
transformation. The specific aims of the present proposal include
the following: (1) Revertants resulting from mutations in different
transformation effector and suppressor genes will be derived
from: (a) populations of v-fos transformed fibroblasts treated with
a variety of mutagens; (b) from populations of cell transformed
with a variety of different oncogenes. (2) The transformation effector genes present in normal cells will
be identified by their ability to induce retransformation of
revertant cells in DNA mediated gene transfer experiments. (3) Dominant transformation suppressing genes will be identified
in revertant cells isolated from populations of tumor cells
transfected with genomic DNA or cDNA's derived from normal
human cells. (4) The transfected transformation effector and suppressor genes
will be molecularly cloned from genomic libraries or cDNA
libraries prepared from the recipient cells, and subjected to
nucleotide sequence analysis. (5) Antisera to each of the cloned effector and suppressor gene
products will be developed using synthetic peptides or proteins
partially purified from bacteria expressing the cDNA clones.
These antisera will be used to study the biochemical properties of
their corresponding proteins, their intracellular localization, as
well as the relative expression and processing in normal versus
transformed cells.
StatusFinished
Effective start/end date5/1/886/30/97

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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