Conservation of Meiotic Recombination Sites in the Human Genome

Project Details

Description

DESCRIPTION (provided by applicant): Meiotic recombination is one of the important biological processes, and may be responsible for many human diseases. In humans, meiotic recombination is known to be clustered mostly at certain "recombination sites" known as recombination hotspots. Although recombination hotspots are often found at the same locations in different human individuals, very little is known about their conservation. In the proposed study, a hypothesis that recombination hotspots in the human genome are highly conserved but are subjected to substantial changes which may be responsible for the generation of new sites, and weakening and extinguishing existing sites. To do so, the short arm of chromosome 18 will be used as the subject for the study. In the first step of the project, 9,000 single sperm samples from three human males, 3,000 each, will be subjected to genotype analysis at 4,000 loci of Single Nucleotide Polymorphisms (SNPs) after whole genome amplification (WGA). This will be done by using a high-throughput genotyping approach developed in the P.I.'s laboratory. Completion of this round of analysis will detect and localize the recombination hotspots to ~4.8 kb regions along length of the chromosomal arm. These spots will be further localized to
StatusFinished
Effective start/end date4/1/081/31/13

Funding

  • National Institutes of Health: $312,000.00
  • National Institutes of Health: $312,000.00
  • National Institutes of Health: $305,791.00
  • National Institutes of Health: $308,880.00

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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