CONTROL OF GENE EXPRESSION IN TUMOR VIRUSES AND CELLS

Project Details

Description

The use of animal viruses as model systems for probing the complexities of
molecular mechanisms has been particularly fruitful. It is generally
thought that the understanding of genetic regulation in viruses will
provide an insight into similar processes in eucaryotic cells.

The overall objective of our research is to systematically develop our
understanding of gene regulation in virus infected cells. This knowledge
will be used for studying the regulation of normal cellular genes and
oncogenes and for developing in-vitro systems from which activities can be
purified and characterized. In the present revised application we
especially intend to accomplish the followings:

1.We shall determine the functions of the carboxylic repeated domain of the
largest subunit of RNA polymerase II in transcription initiation and
elongation.

2.We shall determine the involvement of T-Ag, agnoprotein and RNA secondary
structure in the regulation of transcription attenuation and translation in
SV40.

3.We shall use in vitro transcription of HIV-1 for the analyses of trans
elements involved in transcription attenuation.

4.We shall use in vitro and in vivo transcription directed by the P38
promoter of the parvovirus minute virus of mice (MVM) for the analyses of a
downstream repressor element.

The acquisition of knowledge by the present research will ultimately
contribute to a better understanding of the more complex phenomena in
mammalian cells, such as the molecular processes of differentiation,
development and malignant transformation.
StatusFinished
Effective start/end date12/31/892/28/95

ASJC

  • Genetics
  • Molecular Biology
  • Oncology
  • Cancer Research

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