Development of a discovery platform based on microfluidics and fluorescent cell f

  • Yarmush, Maish (CoPI)
  • Vemula, Muralikrishna (PI)
  • Buehrer, Benjamin (PI)
  • Tilles, Arno (CoPI)
  • Vemula, Muralikrishna (CoPI)
  • Buehrer, Benjamin M. (PI)
  • Tilles, Arno W. (PI)
  • Yarmush, Martin L. (PI)

Project Details


DESCRIPTION (provided by applicant): The overall goal of the project is to develop discovery platform based on microfluidics and functional cell assays that is suitable for screening hundreds of proteins or small-molecule compounds simultaneously in a cost-effective and high throughput manner. The projects Specific Aims are: (1) To develop a microfluidic-based platform for high-throughput screening of potential protein and small molecule therapeutics. (2) To develop an insulin stimulated glucose uptake assay in differentiated 3T3-L1 adipocytes using fluorescent 2-deoxy glucose (2-DOG) analog. (3) To integrate and perform a functional 2-DOG uptake assay in the microfluidics based multi-well cell culture biochip. The deliverable from this completed project is a cost-effective microfluidics based platform suitable for high throughput screening of proteins and small molecule therapeutic compounds.

PUBLIC HEALTH RELEVANCE: The long term objective of the project is to identify protein and small molecule therapeutics to treat diseases such as diabetes, obesity, cancer and neurological disorders. As a first step towards this objective, a platform based on microfluidics technology and functional cell bioassays is being developed to screen the entire human proteome and small molecule libraries in a cost-effective and time saving manner. Presently, safety and efficacy of most of the drugs in the market is still a primary concern. However, with more therapies becoming available, it is possible that not only safety standards will be met but also the cost of drugs will be lowered.
Effective start/end date9/15/118/31/14


  • National Institute of Diabetes and Digestive and Kidney Diseases: $339,072.00
  • National Institute of Diabetes and Digestive and Kidney Diseases: $349,963.00


  • Medicine(all)
  • Oncology
  • Cancer Research
  • Cell Biology


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