Development Of Inhibitors Of P. Falciparum Cgmp Dependent Protein Kinase (Pfpkg) For Malaria Chemoprevention

Description

Malaria is caused by the protozoan parasite, Plasmodium. It begins with the infection by Plasmodiumsporozoites of the liver. This step is essential for the expansion of parasite numbers and the subsequentsymptomatic erythrocytic cycle. Inhibition of pre-erythrocytic infection will prevent malaria pathology andrelapses from P. vivax. Current drugs against pre-erythrocytic stages have significant side-effects or areexpensive. Therefore, there is an urgent need for new drugs against pre-erythrocytic stages of Plasmodium.Our scientific premise is that inhibition of P. falciparum cGMP-dependent protein kinase (PfPKG) will block thepre-erythrocytic cycle, and impede the erythrocytic cycle. Our hypothesis is based on data demonstrating that(i) chemical inhibition of PfPKG prevents infection by P. falciparum sporozoites of tissue culture cells, and by P.berghei sporozoites of mice. (ii) Chemical or genetic inhibition of P. berghei PKG blocks development ofinvaded sporozoites into mature, infectious liver stages.We propose to initiate a medicinal chemistry effort to synthesize and test a new chemical series of PfPKGinhibitors. Validated hits from this series demonstrate in vitro selectivity for PfPKG, over its human homolog, inenzymatic activity assays and are activite against the parasite. We propose a medicinal chemistry effort tooptimize molecules from this series to yield potent and PfPKG-selective compounds with whole-cell activity,acceptable pharmaceutical properties for in vivo testing in mice.
StatusActive
Effective start/end date8/1/177/31/20

Funding

  • National Institutes of Health (NIH)

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Sporozoites
Chemoprevention
Protein Kinases
Malaria
Parasites
Plasmodium
Pharmaceutical Chemistry
Infection
Pharmaceutical Preparations
Cyclic GMP-Dependent Protein Kinases
Liver
Cell Culture Techniques
Pathology