DYNAMICS OF ANTIGEN-ANTIBODY INTERACTIONS

Project Details

Description

The overall objective of the studies outlined in the application
is to obtain a quantitative and fundamental physicochemical
understanding of the interactions of immune complexes (IC) with
immunoadsorbents. Special emphasis will be placed upon (1)
obtaining a basic understanding of the complex interactions in
solution involving antigen, antibody complement, and various other
plasma borne molecules; (2) defining and quantifying the reactions
in solution between IC both reactive and non-reactive towards
complement components; and (3) investigating the reactions
occurring during immunosorption of immune complexes with a variety
of receptor molecules bound to solid supports and with cells which
have receptors for immune complexes. Techniques to be used include
classical and dynamic light scattering. HPLC, radioimmumnoassays,
and electron microscopy. Experiments will be carried out with
model immune complexes and with immune complexes isolated from sera
of feline leukemia virus-infected cats. The proposed research will
enhance our understanding of IC formation and behavior in the
presence of complement and specific blood cells, and may provide
a rational basis for future development and design of
immunoadsorption techniques which may be applied diagnostically or
therapeutically to a variety of disease states.
StatusFinished
Effective start/end date2/1/891/31/94

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $65,461.00
  • National Institutes of Health: $50,474.00
  • National Institutes of Health

ASJC

  • Medicine(all)

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