GENE MAPPING PANELS

Project Details

Description

We are evaluating 29 panels of fluorescently labeled markers located
at approximately 10cM intervals. Each chromosome is covered at this
marker density in 1-4 panels (11-17 loci/panel). Individual markers
are labeled with 1 of 3 different fluorescent dyes, combined after
PCR and run in a single gel lane. Genotypes are obtained for each
locus using Applied Biosystems automated DNA Sequencer, and GENESCAN
analysis, Genotypes, and Excel software. These programs automate the
identification of alleles by distinguishing major peaks from PCR
artifacts and facilitate the export of data in a format suitable for
standard genetic analysis programs. To verify the reported genetic
relationships among individuals involved in gene mapping studies, we
developed software to determine the number of alleles shared among
individuals within a family. We use these statistics to distinguish
full and half sibs and parent-child relations from unrelated
individuals. Finally, we are developing a database using Fourth
Dimension software so that the tremendous amounts of data generated
can be processed efficiently in an integrated suite of specialized
computer programs for linkage/association studies.
StatusNot started

Funding

  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research
  • National Institute of Dental and Craniofacial Research

ASJC

  • Medicine(all)
  • Dentistry(all)
  • Genetics
  • Biochemistry
  • Computer Science Applications
  • Statistics and Probability

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