GENETIC COMPETENCE APPARATUS OF BACILLUS SUBTILLIS

Project Details

Description

Bacillus subtilis can be transformed by exogenous DNA when in a
physiological state known as competence. Competent cells are able to bind
double-stranded DNA, fragment the DNA on the cell surface, and transport
a single strand across the cell envelope layers, with concomitant release
of acid soluble products of the other strand. Our long term objective is
to understand these processes on the molecular level. Previous work has
identified several genes that appear to be necessary for the binding,
processing and/or transport of transforming DNA. In most cases, these genes
have been cloned, sequenced, transcriptionally mapped, and their regulation
studied. With one exception, all of the predicted gene products are
hydrophobic, and likely to be membrane associated. We will use a combined
genetic, biochemical and immunological approach to analyze the properties
of several of these proteins, and to determine their cellular locations.
The comG ORF1 product is similar to that of virB ORF11 of the Agrobacterium
tumefaciens T1-plasmids. The latter product has been shown to be an ATPase.
We will purify the comG ORF1 product determine whether it too is an ATPase,
and if so, use a mutagenic approach to analyzing the role of this enzymatic
activity in several competence-related functions. We will also determine
the cellular location of this protein. Similar experiments will analyze the
role of comG ORF3 in transformation. This product is related to the
Pseudomonas class of bacterial pilins. We will study the possible role in
transformation of a competence-specific single strand binding protein, that
has been described in the literature. We will use a UV cross-linking
approach to isolate a competence-specific DNA binding protein or receptor
complex. Finally, we will utilize electron microscopy to study the cellular
locations and interrelationships of several competence proteins.
StatusFinished
Effective start/end date12/1/902/28/18

Funding

  • National Institutes of Health: $514,676.00
  • National Institutes of Health: $382,239.00
  • National Institutes of Health: $536,135.00
  • National Institutes of Health
  • National Institutes of Health: $390,000.00
  • National Institutes of Health: $576,295.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $397,500.00
  • National Institutes of Health
  • National Institutes of Health: $367,538.00
  • National Institutes of Health: $191,235.00
  • National Institutes of Health: $390,000.00
  • National Institutes of Health: $397,500.00
  • National Institutes of Health: $386,100.00
  • National Institutes of Health: $389,313.00
  • National Institutes of Health: $556,868.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $573,269.00
  • National Institutes of Health: $29,462.00
  • National Institutes of Health: $171,912.00
  • National Institutes of Health
  • National Institutes of Health: $525,744.00
  • National Institutes of Health: $397,500.00
  • National Institutes of Health: $53,000.00

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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