ION CHANNEL DIVERSITY IN PRIMARY-AUDITORY NEURONS

Project Details

Description

With the increased use of cochlear implants in the clinical treatment of
profound sensorineural hearing loss, it has become critical to learn as
much as possible about the nerve being stimulated - the primary-auditory
nerve. There are two major issues that must be examined: the neurons'
electrical properties and their regenerative capacity when deprived of
peripheral targets. A single element that contributes to the regulation
of both of these processes are transmembrane ion channels.

The goal of this project is to determine how diverse groups of ion
channels observed within the primary-auditory neural membrane affect
signal processing and growth regulation. Preliminary studies have shown
that primary-auditory neurons of a lower vertebrate, the goldfish,
possess channels within the internodal membrane that are capable of
rhythmically altering the membrane potential and responding to
extra-synaptic modulation. Yet the functional significance of this
finding is unknown and, furthermore, it has not been determined whether
these specialized membrane channels are also present in mammalian
primary-auditory neurons. Since these channels could alter the duration
and timing of action potentials that propagate along the length of the
neuron, one functional implication is that synaptically generated signals
may be modified on their way into the central nervous system.
Alternatively, these channel types may be transiently incorporated into
primary-auditory neurons for the regulation of processes such as growth
and differentiation.

Patch-clamp recordings will be made from primary-auditory neurons placed
in vitro to examine the elementary properties of their ion channels. The
kinetics, voltage dependence and pharmacological sensitivity of these
channels will be studied to better understand their regulatory effects.
Intracellular recordings will be made to evaluate the contribution of
specific ion channel types to neuronal activity. Other experiments will
be made to categorize electrophysiological changes related to neurite
regeneration or cytodifferentiation.

By using a combination of approaches to study the membrane properties of
primary-auditory neurons, new insights may be gained into the fundamental
mechanisms that regulate cell signaling and differentiation in the
peripheral auditory system.
StatusActive
Effective start/end date12/1/9211/30/21

Funding

  • National Institute on Deafness and Other Communication Disorders: $320,443.00
  • National Institute on Deafness and Other Communication Disorders: $329,375.00
  • National Institute on Deafness and Other Communication Disorders: $335,432.00
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders: $329,375.00
  • National Institute on Deafness and Other Communication Disorders: $353,763.00
  • National Institute on Deafness and Other Communication Disorders: $383,083.00
  • National Institute on Deafness and Other Communication Disorders: $58,941.00
  • National Institute on Deafness and Other Communication Disorders: $345,449.00
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders: $328,716.00
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders: $254,113.00
  • National Institute on Deafness and Other Communication Disorders: $268,286.00
  • National Institute on Deafness and Other Communication Disorders: $261,096.00
  • National Institute on Deafness and Other Communication Disorders: $323,680.00
  • National Institute on Deafness and Other Communication Disorders: $353,763.00
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders: $353,763.00
  • National Institute on Deafness and Other Communication Disorders: $50,000.00
  • National Institute on Deafness and Other Communication Disorders: $13,905.00
  • National Institute on Deafness and Other Communication Disorders: $329,375.00
  • National Institute on Deafness and Other Communication Disorders
  • National Institute on Deafness and Other Communication Disorders: $329,375.00
  • National Institute on Deafness and Other Communication Disorders: $310,189.00
  • National Institute on Deafness and Other Communication Disorders: $310,189.00

ASJC

  • Medicine(all)
  • Neuroscience(all)
  • Speech and Hearing
  • Physiology

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