Project Details


The objective of this project is to develop a bench top laser based stable
isotope analyzer for 13C for the purpose of studying metabolic pathways.
The project will determine the feasibility of developing an analyzer for
13C:12C ratios with at least part per thousand sensitivity using a tunable
laser. One important health related application will be the development of
a simple diagnostic test for the bacterium Heliobacter pylori, which
infects virtually all people with duodenal ulcers and about 80% of those
with stomach ulcers, replacing the radioactive 14C or mass spectrometer
detected 13C labeled urea breath tests. The assay will be based on isotope
shifts in the energy levels of the CO2 molecule. A low pressure (~1 torr)
cell with exhaled breath will be probed by selected isotopic resonance
lines using a low power (~100 mW) tunable laser. Laser transitions and
operating powers will be chosen to enhance the 13C:12C sensitivity with 10-
4 ratios reasonably anticipated. Test cells working with isotopically
labeled CO2,N2,He mixtures will first be studied to optimize sensitivity
and for calibration. Success with the prototype system will then lead to
expanded research on other stable isotope assays and implementation of easy
to operate instrumentation for research and clinical use.
Effective start/end date4/21/9210/20/93


  • National Center for Research Resources


  • Biochemistry, Genetics and Molecular Biology(all)


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.