Project Details

Description

The overall objective of this project is to isolate overlapping
clones representing the euchromatic part of the Drosophila genome.
This will be accomplished in three steps. First, the euchromatic
portions of the salivary gland chromosomes will be microdissected
into regions of approximately 200-300 kb and the genomic DNA from
each region cloned into a lambda bacteriophage vector. This will
yield approximately 600 minilibraries representing part of the
sequence complexity of each interval. Second, the unique sequence
clones in each minilibrary will be used to screen an ordered and
complete library, constructed in a cosmid vector designed for
efficient use of the clones for subsequent chromosomal walking
and/or P-factor mediated transformation experiments. The overlaps
between cosmids from each microdissected region will be established
by restriction mapping. For the majority of regions, it is likely
that the overlapping cosmids will define a single continuous
stretch of genomic DNA (a contig). In addition, many of the
interval contigs representing adjacent microslices will be
connected simply from the restriction maps. Third, given
sufficient time, the remaining gaps will be closed by limited
chromosomal walking between unconnected contigs. This project will
advance substantially an understanding of the structural
organization of the genome of this important model organism and
contribute to the development of techniques important for
characterizing more complex genomes. Furthermore, the clones
obtained will be invaluable to many Drosophilists for studying
specific genes and their environs.
StatusFinished
Effective start/end date7/1/896/30/92

Funding

  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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