MOLECULAR DISSECTION OF SEROTONIN 1C RECEPTOR

Project Details

Description

The proposal of this RCDA application is to study the structure-function
relationship of the serotonin 1c receptor from the mouse brain. As a
neurotransmitter, serotonin exerts diverse effects in the central nervous
system and in periphery by interacting with specific membrane receptors.
These receptors are also the targets of a number of clinically important
drugs as well as several hallucinogenic compounds. The goal of this
proposal is to use the cloned serotonin 1c receptor CDNA for functional
expression of the receptor in Xenopus oocytes and in mammalian cell
culture. This receptor is chosen for study because two unusual aspects: 1)
The receptor contains wight hydrophobic domains, unlike other G protein-
coupled receptors that have seven. Preliminary studies suggest that this
additional domain may serve a functional role in ligand binding; 2) Cells
expressing the receptor respond to changes in external calcium in a manner
similar to serotonin-induced effect, and this takes place only after
pretreatment with serotonin. This therefore suggests a novel mechanism for
receptor-mediated transmembrane signaling. Detailed molecular analysis
will be carried out by generating specific mutations in the receptor, and
electrophysiological and pharmacological methods will be used to
characterize functional modifications. The aim of this proposal relates to the candidate's long-term goal: to
develop a research career in the field of neurobiology by using the
expertise in molecular biology and electrophysiology to study fundamental
questions in neurobiology. The award would permit he candidate to devote
blocks of uninterrupted time to further his knowledge in areas important to
his career goal: auditing classes in neurobiology and acquiring hands-on
experience in serotonin receptor pharmacology.
StatusFinished
Effective start/end date7/1/926/30/97

Funding

  • National Institutes of Health: $69,947.00
  • National Institutes of Health: $73,327.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $74,412.00

ASJC

  • Medicine(all)
  • Neuroscience(all)

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