MOLECULAR DISSECTION OF THE SEROTONIN 1C RECEPTOR

Project Details

Description

The neurotransmitter serotonin exerts diverse physiological effects in the
central and peripheral nervous systems and in smooth muscle by interacting
with specific membrane receptors. Serotonin receptors are the targets of
many clinically important drugs. These receptors are classified into
pharmacologically distinct subtypes, and some of the subtypes have been
cloned recently. This proposal is for a molecular analysis of one subtype,
the serotonin 1c receptor from the mouse brain. The receptor mRNA will be
synthesized from the cDNA clones by in vitro transcription and will be
injected into Xenopus oocytes for functional expression.
Electrophysiological methods will be used to characterize the receptor
function and to examine the changes in its function resulted from
structural modifications. Several drugs, some of which are used clinically for neurological and
psychiatric treatments, will be used to study their effects on receptor
function. The possibility of a novel mechanism for the receptor-effector
coupling will also be examined. In addition, the extra hydrophobic domain
of the receptor, a unique feature of this receptor of the 17-helix" G
protein-coupled receptor family, will be analyzed in detail to determine
its functional significance. Also will be studied are the receptor
desensitization and its influence on the receptor-mediated signal
transduction. These studies will establish an understanding of the function
of the serotonin 1c receptor at the molecular level, and will contribute to
our knowledge of the receptor's role in bodily function.
StatusFinished
Effective start/end date9/17/908/31/95

Funding

  • National Institutes of Health: $92,915.00
  • National Institutes of Health: $83,845.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Neuroscience(all)

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