Project Details


Determining the detailed structure and nucleotide sequence of chromosomes
from model eucaryotic organisms will be important for understanding how
chromosomes function to express, replicate and transmit genetic
information. It is anticipated that this information will have important
consequences for understanding human chromosome function and gaining
knowledge about genetic disease. The yeast, Saccharomyces cerevisiae is
one of the most well characterized model organisms and has the advantages
of having chromosomes that both are relatively small and behave like the
chromosomes of higher organisms. Chromosome I is the smallest S.
cerevisiae chromosome and contains a DNA molecule that is only 240 kb
making it the smallest normal eucaryotic chromosome yet found. Virtually
all of chromosome I is cloned; transcription has been analyzed on
two-thirds of it and deletion mutations have been produced in
approximately half of its 90-100 genes. In addition, the genetic and
physical maps for 90% of the DNA molecule have been aligned and reveal
that similar to small human chromosomes, this chromosome undergoes high
rates of meiotic recombination. These studies make chromosome I one of
the best characterized yeast chromosomes. We now propose to determine
the entire nucleotide sequence of chromosome I in collaboration with a
consortium of 7 Canadian laboratories as mandated by the International
yeast community. Conventional methodology will be used throughout due to
the small size of the DNA molecule. Sequences will be analyzed for
transcripts, open reading frames, proteins homologies, origins of DNA
replication, transposons, etc. Based on the number of genes that
currently are mutationally defined on this chromosome, this project will
provide fast and important information for relating sequence to
biological function and provide a paradigm for the study of other larger
Effective start/end date1/1/9312/31/95


  • National Institutes of Health: $79,386.00
  • National Institutes of Health
  • National Institutes of Health: $74,043.00


  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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