NEUROPHYSIOLOGY OF SPINAL CORD INJURY

Project Details

Description

We seek to continue our studies of calcium (Ca) changes in spinal cord
injury. The proposed experiments are based on our recent findings of large
extracellular and tissue Ca changes in cat spinal cords contused by a
standard weight drop. The experiments seek to define the relationships of
Ca changes with axonal morphology, tissue ATP and phosphate levels, and
losses of membrane integrity as demonstrated by axonal uptake of
horseradish peroxidase (HRP).

A four year study is envisioned. In the first year, we will localize and
quantify spinal cord Ca by atomic absorption spectroscopy, ion-selective
microelectrodes, and histological methods. In the second year, the
measured Ca changes will be correlated with axonal morphology, tissue ATP
and phosphate, and distribution of axonal uptake of HRP. In the remaining
years, we will investigate a newly discovered phenomenon, that pulse
electromagnetic fields (PEMF) specifically alter Ca distribution in injured
spinal cords without changing tissue Na, K, or water. The techniques
utilized and developed in the preceding two years will be applied to define
the specific effects of PEMF on spinal cord Ca, phosphates, ATP, and axonal
morphology.

By the end of the proposed work, we expect to have determined: (1) the
routes and destination of Ca ion shifts in our spinal injury model; (2) the
extent to which Ca precipitates with phosphates inside axons; (3) the
effect of Ca changes on tissue phosphates and ATP in injured cords; (4) the
relationship of Ca accumulation to axonal morphological changes; (5) the
proportion of Ca localizing in intact axons not penetrated by HRP; (6) the
precise Ca distribution changes produced by PEMF in spinal injury; (7) the
acute effects of PEMF on tissue phosphates, ATP, axonal morphology, and HRP
uptake by axons in contused spinal cords.
StatusFinished
Effective start/end date12/31/8912/31/97

Funding

  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke
  • National Institute of Neurological Disorders and Stroke

ASJC

  • Medicine(all)
  • Neuroscience(all)
  • Clinical Neurology
  • Neurology
  • Physiology

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