Optical Scatter Microscopy

Project Details


DESCRIPTION (Adapted from the applicant's abstract): Although alterations in
the shape and size of subcellular organelles are important markers of cell
death, these important morphological changes cannot be directly quantified in
living cells by conventional light microscopy. Quantitative light scattering
provides an appealing solution to this problem. This technique is
noninvasive, and is sensitive to the dimensions of particles with size on the
order of the wavelength. Existing light scattering methods, which have been
successfully utilized in flow cytometry, are spectroscopic in nature and do
not permit sample imaging. Preserving spatial information is crucial to
correlating the source of the scatter changes with a given cell or organelle.
To this end, the investigators propose to combine light scattering
spectroscopy with imaging. This novel technology, OSM, will produce the first
images in which alterations in organelle morphology can be tracked in living
cells in real time.

The preliminary data attest to the feasibility and sensitivity of OSM.
Notably, the investigators were able to detect an intracellular optical
scatter change early during apoptosis, long before it was discernable by other
light microscopic methods, such as DIC. However, the biological events
responsible for this scatter change remain to be identified. Elucidating the
relationship between the optical scatter changes and cellular activity is the
principal challenge in developing OSM. If this challenge is met, OSM has the
potential to transform significantly the approach to non-invasive monitoring
of intracellular activity, and holds promise to fundamentally enhance high
resolution light microscopy.

In this proposal, the investigators present a rational strategy and step-by-
step approach to implement and validate OSM. In this initial phase of
development, the investigators will use OSM to study mitochondrial dysfunction
during cell death. The long-term goal is to develop OSM as a tool for
functional microscopy.
Effective start/end date7/1/016/30/03


  • National Center for Research Resources: $118,561.00
  • National Center for Research Resources: $122,625.00


  • Cell Biology


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