PACKAGING OF THE SEGMENTED GENOME OF BACTERIOPHAGE O6

Project Details

Description

Bacteriophage Phi6 contains three pieces of double-stranded RNA in its
nucleocapsid. The pieces contain different genetic information but have
similar termini. The aim of this proposal is to determine how the
packaging system is able to select one of each of the chromosomes.
Specifically, the regions of the three chromosomes necessary for packaging
will be identified. The order of packaging and the requirements for
packaging in terms of amount of RNA will be determined. The studies will depend upon the use of cDNA clones of the Phi6
chromosomes. Chimeras and other constructions of the cDNA clones will be
prepared and the packaging of the transcripts of the cDNA will be studied.
In addition, cDNA clones of the large chromosome will be prepared. The
products of this chromosome should form procapsids capable of packaging RNA. The nature of the mechanisms of selective packaging in viruses of segmented
genomes is currently unknown. The elucidation of the mechanism for Phi6
will probably be relevant to packaging of other segmented genome viruses.
StatusFinished
Effective start/end date1/1/856/30/10

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $335,008.00
  • National Institutes of Health
  • National Institutes of Health: $426,672.00
  • National Institutes of Health: $426,754.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $359,834.00
  • National Institutes of Health: $349,353.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $153,415.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $423,433.00
  • National Institutes of Health
  • National Institutes of Health: $272,228.00
  • National Institutes of Health: $370,630.00
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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