PARACRINE CONTROL OF FOLLICULAR GROWTH AND OVULATION

Project Details

Description

The long term objective of this research is to understand the fine controls
of ovarian function and thus to provide opportunities to control fertility
and alleviate some forms infertility in women. The present dimension is to
understand the paracrine control of follicular development and ovulation
through the study of the local production and some of the biological
actions in common between prostaglandins (PGs) and relaxin in the follicle
of the pig and rat. The distribution of their sites of production and
their receptors in situ in pig and rat follicles will be determined
throughout follicular development and after inhibition of ovulation. The
factors modulating this local production of PGE2, PGF2Alpha and relaxin by
isolated granulosa and theca cells as well as follicle wall will be studied
in vitro. The endogenous release of paracrine relaxin into porcine
follicular fluid will be deduced from measurements by RIA and compared with
PGE2 and PGF2Alpha levels during follicular development. Selected biological actions in common between PGE2, PGF2Alpha and relaxin,
the release of enzymes involved in collagen remodelling, will be related to
the localization of their respective receptors in the follicle during
development. Methodology will include the determination of the sites of
hormone production in situ by immunocytochemistry and hormone receptor
localization by quantitative light microscopic autoradiography. Hormone concentrations in follicular fluids and in media from in vitro
cultures of follicular tissue will be measured by conventional
radioimmunoassay. The biological actions of the prostaglandins and relaxin
in common will be determined by measuring the release of plasminogen
activator and collagenase by isolated cells in vitro.
StatusFinished
Effective start/end date7/1/8512/31/91

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $90,711.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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