PHOSPHOLIPID METABOLISM &MEMBRANE FUNCTION

  • Carman, George (PI)
  • Carman, George (PI)
  • CARMAN, GEORGE (PI)
  • CARMAN, GEORGE M. (PI)
  • CARMAN, GEORGE M. (PI)
  • CARMAN, GEORGE M. (PI)
  • CARMAN, GEORGE (PI)

Project Details

Description

The membrane-associated phospholipid biosynthetic enzymes are important
because their activities are involved in structural as well as functional
properties of cell membranes. In the yeast Saccharomyces cerevisiae the
enzyme activities in the reaction sequence phosphatidic acid - greater than
CDP-diacylglycerol - greater than phosphatidylserine - greater than
phosphatidylethanolamine - greater than phosphatidylmonomethylethanolamine
- greater than phosphatidyldimethylethanolamine - greater
phosphatidylcholine are coordinately regulated to the synthesis of
phosphatidylinositol. This application will focus on the regulation of
four of the enzyme activities in this pathway, namely, CDP-diacylglycerol
synthase, phosphatidylserine synthase, phosphatidylinositol synthase, and
the phospholipid N-methyltransferases. CDP-diacylglycerol synthase and the
phospholipid N-methyltransferases will be purified to homogeneity and will
be characterized with respect to enzymological, chemical, and physical
properties. Purified preparations of phosphatidylserine synthase and
phosphatidylinositol synthase are already available for the project. The
purified enzymes will be reconstituted into well defined unilamellar
phospholipid vesicles to study activity modulation by phospholipids,
water-soluble metabolites, and by phosphorylation/dephosphorylation.
Antibodies specific for each of the enzymes will be prepared and used as
probes to study the regulation of enzyme formation in wild type and mutant
strains of S. cerevisiae grown under various conditions. Enzyme regulation
will also be studied in clones containing the structural genes encoding for
these enzymes. The results found with S. cerevisiae should be relevant to
higher eucaryotic organisms.
StatusActive
Effective start/end date12/31/896/30/21

Funding

  • National Institute of General Medical Sciences: $402,039.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $354,435.00
  • National Institute of General Medical Sciences: $255,106.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $289,633.00
  • National Institute of General Medical Sciences: $402,039.00
  • National Institute of General Medical Sciences: $402,039.00
  • National Institute of General Medical Sciences: $402,039.00
  • National Institute of General Medical Sciences: $352,771.00
  • National Institute of General Medical Sciences: $249,015.00
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences: $351,897.00
  • National Institute of General Medical Sciences

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Chemistry(all)
  • Molecular Biology
  • Biochemistry
  • Catalysis
  • Cell Biology

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