Project Details


We will pursue aspects of the molecular biology of pE194, a 3500 base pair
plasmid in Bacillus subtilis which confers resistance inducibly to the
macrolide-lincosamide-streptogramin B antibiotics. The resistance is conferred
via a specific N-methylation of adenine in 23S ribosomal RNA. We will continue
studies on the post-transcriptional regulation of synthesis of a protein
required for this resistance. Regulation is accompanied by adjustments in the
stability of the mRNA for this protein. We will purify and characterize an rRNA
methylase specified by pE194. We will pursue studies on gene-overlap in the
pE194 genome. These studies include DNA, protein and RNA sequencing and the
study of plasmid mutants. Finally, we will continue to characterize DNA
replication control in pE194 focussing on defining and studying those regions of
the genome which are required for replication and to produce a plasmid-specific
trans-acting replication substance. We hope to eventually sequence the entire
plasmid genome.
Effective start/end date12/1/8011/30/85


  • National Institutes of Health


  • Medicine(all)
  • Immunology and Microbiology(all)

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