PRENATAL DIFFERENTIATION OF THE PITUITARY GLAND

Project Details

Description

This is a proposal to study specific aspects of prenatal pituitary gland
development. Our long-range goal is to determine the role that tissue
interactions have in establishing lobe-specific biochemical differences
between adult anterior and intermediate lobes of the pituitary gland. We
will study differentiation of the two populations of proopiomelanocortin
(POMC)-containing cells that are present in the anterior and intermediate
lobes of the pituitary gland. Although POMC cells in both lobes make a
similar precursor protein molecule, the precursor is processed in the adult
animal to different lobe-specific end-product hormones. We will determine
when and how during development these lobe-specific processing patterns are
established. At the genetic level, we will use recombinant DNA probes to
determine when POMC gene expression can first be detected and compare this
to the time at which POMC-related peptides are first detected
immunologically. We will also use these probes to localize POMC-producing
cells in embryonic pituitary tissue sections at different times of
development and establish techniques to examine the possible dual
production of peptide hormone mRNAs in single cells during development. We
will determine whether other proteins which exhibit lobe-specific
localization in the adult pituitary are early markers of lobe-specific
differentiation. We have determined that monoamine oxidase B distribution
in the adult rat pituitary is lobe-specific and will extend this analysis
to early fetal stages. Finally, we will begin to characterize the
extracellular matrix (ECM) in the region of the developing pituitary and
determine the role of ECM in establishing lobe-specific differentiation by
in vitro culture studies.
StatusFinished
Effective start/end date8/1/834/30/93

Funding

  • National Institutes of Health
  • National Institutes of Health: $208,443.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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