PROTEIN FATTY ACYLESTERASE IN SALIVARY GLANDS

Project Details

Description

Salivary glycoproteins, the major constituent of saliva, play important
roles in the protection of soft oral tissue and teeth from ulcerations,
abrasions and caries. This task is accomplished when glycoprotein is
sufficiently acylated thus capable to form complexes with lipids and
proteins of saliva to adhere to oral surfaces and to repel proteolytic
attacks of hostile environments. According to our recent reports, the
covalently linked fatty acyl residues are added to the protein core of
glycoproteins during co-post translational modifications and that the
acylating enzyme responsible for the addition of these fatty acyl
residues, is a rough endoplasmic reticulum (RER) origin, having its
catalytic site exposed to the cytosolic side of RER. However, the
understanding of the enzyme protein fatty acylesterase (PFAE)
responsible for the removal of the fatty acyl residues, which may alter
the net content of fatty acyl residues present in glycoproteins and
which may modify its function and its susceptibility to protease, is
very limited. Variations in the content of covalently linked fatty
acids found in the salivary glycoproteins of caries resistant and
susceptible individual provide indirect evidence suggesting that the
acylesterase may be involved in modulation of secretory proteins and its
altered activity is evident in pathological conditions with symptoms of
scanty but precipitously thick saliva. Thus the present project is
directed to explore the presence of PFAE in saliva and in salivary
glands. The approach to the project is to identify the subcellular
fraction of a salivary cell having maximal PFAE activity and to rule out
the possibilities that PFAE activity is different than protein fatty
acyltransferase and Lipid Lipases. Then our aim is to determine the
topology of PFAE in RER using mild trypsinization of RER. The findings
from this project should improve our understanding of the synthesis and
processing of acylated proteins and should lead to the study on the
regulation of PFAE activity under pathological conditions.
StatusFinished
Effective start/end date5/1/914/30/94

Funding

  • National Institutes of Health: $121,750.00

ASJC

  • Medicine(all)
  • Dentistry(all)

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