REGULATION OF PHOSPHATIDYLINOSITOL METABOLISM

Project Details

Description

Phosphatidylinositol metabolism is important to the growth of eukaryotic
organisms. The agonist-stimulated hydrolysis of membrane
polyphosphoinositides in animals result in the stimulation of various
cellular responses. The enzyme that catalyzes the initial step in the
synthesis of the polyphosphoinositides is phosphatidylinositol kinase.
This application will focus on the regulation of phosphatidylinositol
kinase in the simple eukaryote, Saccharomyces cerevisiae.
Phosphatidylinositol kinase will be purified to homogeneity and will be
characterized with respect to enzymological, chemical, and physical
properties. The purified enzyme will be reconstituted into well defined
unilamellar phospholipid vesicles to study the regulation of activity.
Regulation will be studied by altering the composition and environment of
the vesicles and by possible phosphorylation and dephosphorylation of the
enzyme. Antibodies specific for phosphatidylinositol kinase will be
prepared and used as a probe to study the regulation of
phosphatidylinositol kinase formation in cells grown under various
conditions. The proposed studies should provide insight into the
regulation of polyphosphoinositide synthesis. The results found with S.
cerevisiae should be relevant to higher eukaryotic organisms.
StatusFinished
Effective start/end date12/31/896/30/95

Funding

  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Cell Biology

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