Lipids form the structural basis for biological membranes (e.g., phospholipids) and are stored in lipid droplets (e.g., triacylglycerol) as an energy reserve. Their synthesis also prevents toxicity caused by free fatty acids that are consumed in the diet. Defects in the metabolism of lipids lead to a host of diseases such as lipodystrophy, obesity, heart disease, peripheral neuropathy, myoglobinuria, and inflammation. This project focuses on phosphatidate phosphatase, a key enzyme that controls the proportional synthesis of triacylglycerol and membrane phospholipids. Too much activity results in obesity, whereas too little activity results in lipodystrophy. Thus, the goal of the project is to understand how the expression of the enzyme is regulated and how the activity of existing enzyme is regulated. The work will be addressed through biochemical and molecular studies using the simple baker's yeast as a model system. The ultimate goal is to develop practical means to control and/or treat the obese condition in humans.
|Effective start/end date||10/1/11 → 9/30/16|
- National Institute of Food and Agriculture (National Institute of Food and Agriculture (NIFA))