Project Details

Description

In the yeast Saccharomyces cerevisiae, CTP is a precursor of all membrane
phospholipids synthesized by the phosphatidylethanolamine methylation
(primary) and CDP-ethanolamine- and CDP-choline-based (auxiliary)
pathways. This grant application addresses the hypothesis that cellular
levels of CTP regulate the synthesis of phospholipids by the primary and
auxiliary pathways by controlling the expression and/or activities of the
enzymes in these pathways. The effects of CTP on phospholipid synthesis
will be examined in vivo using S. cerevisiae strains expressing various
levels of CTP. CTP levels will be varied through the expression of the
URA7 gene, which encodes CTP synthetase. The mRNA abundance and protein
levels of phospholipid biosynthetic enzymes will be examined in response
to various levels of CTP. Biochemical regulation of enzyme activities in
response to CTP will also be examined. Since cellular CTP levels must be
governed by the expression and/or activity of CTP synthetase. This
application will also address if CTP synthetase activity is regulated by
factors known to regulated phospholipid synthesis. CTP synthetase will be
purified by molecular genetic and/or biochemical approaches for the
purpose of preparing anti-CTP synthetase antibodies for regulation
studies. The purified enzyme will be characterized with respect to its
basic enzymological and kinetic properties. The regulation of CTP
synthetase by cAMP-dependent protein kinase phosphorylation will be
examined in vivo and in vitro. The information that will be obtained from
these studies should be of great use to, and complement the work of other
investigators in the field of phospholipid metabolism as well as those
working on nucleotide metabolism.
StatusActive
Effective start/end date5/1/946/30/21

Funding

  • National Institutes of Health: $307,114.00
  • National Institutes of Health: $265,437.00
  • National Institutes of Health: $333,250.00
  • National Institutes of Health: $316,407.00
  • National Institutes of Health: $333,250.00
  • National Institutes of Health: $319,974.00
  • National Institutes of Health: $265,437.00
  • National Institutes of Health: $226,699.00
  • National Institutes of Health: $307,114.00
  • National Institutes of Health: $307,114.00
  • National Institutes of Health
  • National Institutes of Health: $267,135.00
  • National Institutes of Health: $118,906.00
  • National Institutes of Health: $313,606.00
  • National Institutes of Health: $267,010.00
  • National Institutes of Health: $274,336.00
  • National Institutes of Health
  • National Institutes of Health: $281,336.00
  • National Institutes of Health: $265,437.00
  • National Institutes of Health
  • National Institutes of Health: $313,606.00
  • National Institutes of Health: $333,250.00
  • National Institutes of Health: $265,437.00
  • National Institutes of Health: $224,953.00
  • National Institutes of Health: $233,412.00
  • National Institutes of Health: $307,114.00
  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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