Project Details


This project proposes to dissect the molecular mechanisms that
determine the pattern of expression of the Drosophila white gene
in specific tissues, specific cells and specific developmental
stages and modulate it according to the dosage of X chromosome
material present in the embryo. The sequence of the different
tissue specific determinants in the white regulatory region, their
relationship to one another, to the promoter and to the zeste
binding sites will be studied using a variety of transposon
constructions introduced into the genome by germ line
transformation. The transformed lines will be tested for the
tissue and cell specificity of expression and for the effects of
zeste and zeste mutations both on the functioning of the white
promoter and on interchromosomal transvection-like effects. In
particular, these experiments will permit the testing of current
hypotheses on the role of the zeste product in governing the
interaction between regulatory sequences and the promoter.

Similar transposon contructions will identify sequences in the
white regulatory region that are able to confer X-chromosome
dependent dosage compensation on gene expression. This will be
done using DNA segments from white placed in front of the
xanthine dehydrogenase gene. Affinity chromotography will then
be used to isolate a protein factor that binds specifically to the
dosage compensation determinant.

This work will test basic mechanisms underlying the functional
organization of genetic information. These mechanisms could
lead to new insights on the control of larger genetic domains such
as the globin gene cluster in man.
Effective start/end date7/1/906/30/91


  • National Institute of General Medical Sciences


  • Genetics


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.