Scientific Core Mass spectrometer (MS)-based proteomic approaches are playing an increasingly importantrole in neuroscience research. However, methods and instrumentation for MS are evolvingextremely rapidly thus the ability to evaluate, then selectively adopt and implement newmethods and technologies is critical. Most laboratories lack the equipment and expertise to dothis, thus the Scientific Core will provide cost-effective and cutting-edge proteomics capabilitiesto NINDS-supported laboratories at Rutgers University. Part of this service will be to provideaccess to two newly-acquired Thermo Q Exactive Hybrid Quadrupole-Orbitrap MSs. This is ahigh-sensitivity/resolution, high-throughput instrument that will be extremely important forneuroscience research with excellent capabilities in protein identification and quantitation,analysis of post-translational modification and biomarker discovery and validation. Even withuniversity-wide subsidies in the existing Cores, MS is expensive and financial factors are adeterrent from optimum utilization of this powerful technology. For example, investigatorsfrequently economize at the expense of optimal experimental design (e.g., analysis of propercontrols) and many large scale MS studies specifically required in neuroscience (e.g., biomarkeridentification) are prohibitive for individual researchers. A key goal of this proposal is to alleviatethis restriction for NINDS-sponsored researchers. The two Co-PI's, Drs. Hong Li and Peter Lobel, are experienced proteomics researchers.As such, they and their respective groups will fulfill a critical role for the Scientific Core inproviding input into experimental design and execution that will be key to success ofproteomics-based neuroscience studies. One aspect of this will lie in the development ofproteomic technologies that are customized for neuroscience research. Finally, analysis of MSdata, in particular ascertaining the significance of changes in protein expression or post-translational modification in quantitative experiments, is challenging thus another key role of theScientific Core will be the contributions of biostatisticians and bioinformaticians with experiencein analyzing MS data, in the analysis of NINDS-supported projects. Finally, increased access to advanced proteomics technology will enhance the researchprograms of individual investigators but will also promote interaction between laboratories. Oneimportant area of collaboration that will be facilitated by the MSCINR will be between basic andclinical researchers in the design and implementation of biomarker studies for multiple sclerosis,Alzheimer's and Parkinson's disease and other neurological diseases.
|Effective start/end date||7/1/15 → 6/30/16|
- National Institutes of Health (NIH)
Design of experiments