Project Details

Description

Better comprehension of the processes involved in the maintenance of oral
health requires the understanding of the salivary components responsible
for the protection of hard and soft oral tissues from physical, chemical
and microbial damage. It is believed now that salivary secretory proteins
play significant role in the maintenance of oral health. Many protective
properties of salivary secretory proteins are acquired through the post-
translational modifications such as sulfation and acylation. Although
sulfation has been well accepted for its significant role in the
processing of secretory proteins, no investigation has so far been
directed towards its regulation. Thus, our major effort in this project is
focussed on the regulation of tyrosine sulfation and glycoprotein
sulfation in salivary glands. Since lipids are the structural components
of biological membranes and that the two sulfotransferases involved in the
sulfation of salivary proteins are integral membrane proteins of Golgi,
the effect of lipids on sulfotransferases will be investigated. Our
preliminary studies suggest that the charges present in lipids could be
responsible for their effectiveness, we will investigate the effect of
polyelectrolytes on sulfotransferases. The effect of sphingosine on the
secretion of salivary proteins will be studied in the isolated salivary
cells. Furthermore, since sphingosine has been shown to regulate the
activities of protein kinase C and phospholipase, in addition to its
action on sulfotransferases, specific inhibitors of these two enzymes will
be used to differentiate the sphingosine regulation of sulfation from its
effect mediated through the changes in those two enzymes.The results of
this project should help in understanding the regulation of the
sulfotransferases involved in salivary sulfoprotein processing which could
be related to sulfoproteins in the pathology of oral disease.
StatusFinished
Effective start/end date9/30/939/29/95

Funding

  • National Institutes of Health
  • National Institutes of Health: $39,813.00

ASJC

  • Medicine(all)
  • Dentistry(all)

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