TB detection in children using a novel stool-based GeneXpert assay

Project Details

Description

Modified Public Health Relevance Section It can be very difficult to diagnose tuberculosis in children since they do not easily cough up sputum where tuberculosis is usually detected. Instead, we think that Mycobacterium tuberculosis bacteria end up in children’s stool, because they swallow their sputum after coughing. This project will develop a simple method to rapidly detect tuberculosis in children by examining their stool samples with a simple and rapid test. Rapid detection of tuberculosis will save many lives by enabling more rapid treatment for this disease. NEW We will optimize, test and validate buffers and methods for the Cepheid Xpert Xpress SARS CoV2 and the Xpert Xpress SARS CoV2/Flu/RSV point of care tests for use with different patient samples. Work to optimize individual and pooled saliva testing for both the uniplex and 3 plex cartridges is time critical and essential to addresses this major threat to public health. The improvements that we make to these assays will allow a more rapid and more widely available testing response to the emerging epidemic.
StatusFinished
Effective start/end date12/5/1711/30/22

Funding

  • National Institute of Allergy and Infectious Diseases: $536,775.00
  • National Institute of Allergy and Infectious Diseases: $448,870.00
  • National Institute of Allergy and Infectious Diseases: $528,819.00
  • National Institute of Allergy and Infectious Diseases: $192,373.00
  • National Institute of Allergy and Infectious Diseases: $549,263.00

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