Project Details

Description

The v-rel oncogene of avian reticuloendotheliosis virus strain T (Rev-T), a
highly oncogenic retrovirus, transforms chicken spleen and bone marrow
cells in vitro and induces lethal B cell lymphomas in vivo. The mechanism
by which the v-rel oncoprotein transforms cells has yet to be determined.
Accumulating evidence suggest that its cellular homolog, c-rel, might
contribute to lymphoid cell differentiation. The recent demonstration that
the mouse and human transcription factors NFkB and KBF1 belong to the
neoplastic transformation and lymphoid cell differentiation. The work described in this proposal will extend our studies on the trans-
acting function of the v- and c-rel proteins by investigating the
functional relationship between the products of the rel gene-family and
transcription factor NFkB. Experiments are presented to define sequences
responsive to the trans-acting function of rel and to map the domains of
the rel proteins involved in DNA-binding, dimerization, association with
cellular proteins and transactivation. The interplay between the trans-
acting functions of the v- and c-rel proteins will also be examined, to
clarify the mechanism by which v-rel and c-rel proteins will also be
examined to clarify the mechanism by which v-rel transforms lymphoid cells.
Finally, we will examine whether and how the protein-protein interactions
in which v- and c-rel participate in lymphoid cells might regulate their
subcellular localization and function. It these experiments indicate that
the interaction of the v- and c-rel proteins with one or more specific
cellular proteins plays a role in their biological effects, our future
research will be directed towards cloning and characterizing the genes that
encode them. These studies will not only help to define the mechanism by
which v-rel transforms lymphoid cells but they will also help to clarify
the function of the c-rel proto-oncogene in lymphoid cell differentiation.
StatusFinished
Effective start/end date7/1/916/30/07

Funding

  • National Institutes of Health
  • National Institutes of Health: $207,103.00
  • National Institutes of Health: $84,117.00
  • National Institutes of Health
  • National Institutes of Health: $265,542.00
  • National Institutes of Health: $231,486.00
  • National Institutes of Health: $265,845.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $223,849.00
  • National Institutes of Health: $265,220.00
  • National Institutes of Health
  • National Institutes of Health: $265,696.00
  • National Institutes of Health: $265,383.00

ASJC

  • Medicine(all)

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