TROPOMYOSINS IN NORMAL AND TRANSFORMED CELLS

Project Details

Description

This research is aimed at an analysis of the functional roles of
tropomyosin in the structure and organization of microfilament bundles in
normal and transformed animal cultured cells. We found that: (1)\of five
forms of tropomyosin found in "normal" rat cell lines, three are major
tropomyosins and two are relatively minor tropomyosins; (2)\upon
transformation, the level of one or both of the major tropomyosins is
decreased and the levels of minor tropomyosins are greatly increased;
(3)\the degree of these changes in tropomyosin patterns correlates well
with the extent of morphological transformation; (4)\the changes in
tropomyosin patterns were also observed in temperature shift experiments
with rat-1 cells transformed with a ts Rous sarcoma virus mutant; (5)\the
changes in the pattern of tropomyosin expression are regulated at the
level of mRNA ratherthan by post-translational modification; and
(6)\changes in tropomyosin patterns are consistently expressed with a
variety of transformation agents including virus, chemical carcinogen,
UV-irradiation, and DNA transfection. These results imply that this
differential expression of tropomyosin between "normal" and transformed
cells may be involved in the changes in actin cables associated with
morphological transformation. To explore this possibility, we have
purified each isoform of tropomyosins from rat cultured cells and will
examine in vitro physiological properties of microfilaments with different
sets of tropomyosin by various biochemical and biophysical methods. In
vivo functional roles of tropomyosin will be determined by microinjection
of different forms of tropomyosin or monoclonal antibodies into living
cells. The long-term goal of these studies is to understand how
differential expression of tropomyosin effects cell morphology changes
upon oncogenic transformation and how actin cables rearrange in this
process. (L)
StatusFinished
Effective start/end date12/31/894/30/11

Funding

  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute: $366,539.00
  • National Cancer Institute: $344,069.00
  • National Cancer Institute: $368,840.00
  • National Cancer Institute
  • National Cancer Institute: $376,564.00
  • National Cancer Institute
  • National Cancer Institute: $365,683.00
  • National Cancer Institute
  • National Cancer Institute: $352,310.00
  • National Cancer Institute: $366,780.00
  • National Cancer Institute
  • National Cancer Institute: $358,177.00
  • National Cancer Institute
  • National Cancer Institute: $347,823.00
  • National Cancer Institute
  • National Cancer Institute: $365,711.00
  • National Cancer Institute
  • National Cancer Institute

ASJC

  • Medicine(all)
  • Genetics
  • Cancer Research
  • Cell Biology

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