Project Details


The research project will focus on the structure and regulation of the
gene encoding tryptophan hydroxylase (TPH), the rate-limiting enzyme in
the synthesis of serotonin. While TPH is known to be regulated through
protein kinase-mediated phosphorylation, this key enzyme may also be
regulated at the level of gene expression. Using oligonucleotide probes
based on cloned, pineal cDNAs encoding TPH, we can detect mRNA from the
CNS. We have shown that the CNS mRNA is altered at the 5' end when
compared with the cloned, pineal cDNAs. Furthermore, we have shown that
pharmacological agents which alter serontonin levels alter steady-state
TPH mRNA levels. We now propose to obtain genomic- and CNS-specific cDNA-
clones specific for TPH and to study the mechanism of gene regulation.
Lastly, we will study the potential involvement of receptor events in the
regulation of TPH mRNA levels.
Effective start/end date8/1/907/31/95


  • National Institutes of Health
  • National Institutes of Health: $62,875.00
  • National Institutes of Health: $90,443.00
  • National Institutes of Health: $77,986.00
  • National Institutes of Health


  • Medicine(all)

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