α-defensin inhibits influenza virus replication by cell-mediated mechanism(s)

Mirella Salvatore, Adolfo García-Sastre, Piotr Ruchala, Robert I. Lehrer, Theresa Chang, Mary E. Klotman

Research output: Contribution to journalArticle

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Abstract

The innate immune system mounts the first host response to pathogens. Because α-defensins, which are cationic antimicrobial peptides of polymorphonuclear neutrophils and other leukocytes, are important effectors of the innate immune system, we studied the antiviral activity of human α-defensin-1 (also known as "human neutrophil peptide-1" [HNP-1]) against influenza virus in vitro. Treatment of cell cultures with HNP-1 soon after infection resulted in marked inhibition of influenza virus replication and viral protein synthesis. This effect was not due to cytotoxicity or to a direct effect on the virus. Treatment of cells with HNP-1 followed by its removal before infection also inhibited viral replication, suggesting that the inhibition was due to the modulation of cellular pathways. HNP-1 treatment inhibited protein kinase C (PKC) activation in infected cells, suggesting the involvement of the PKC pathway. Our data expand the previously known activity of α-defensins against influenza virus. Characterizing the mechanism of action of α-defensins may lead to the identification of new strategies for prevention and therapy.

Original languageEnglish (US)
Pages (from-to)835-843
Number of pages9
JournalJournal of Infectious Diseases
Volume196
Issue number6
DOIs
StatePublished - Sep 15 2007

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Defensins
Virus Replication
Orthomyxoviridae
Protein Kinase C
Immune System
Antimicrobial Cationic Peptides
Viral Proteins
Infection
Human Activities
Antiviral Agents
Neutrophils
Leukocytes
Cell Culture Techniques
Viruses
human neutrophil peptide 1

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Salvatore, M., García-Sastre, A., Ruchala, P., Lehrer, R. I., Chang, T., & Klotman, M. E. (2007). α-defensin inhibits influenza virus replication by cell-mediated mechanism(s). Journal of Infectious Diseases, 196(6), 835-843. https://doi.org/10.1086/521027
Salvatore, Mirella ; García-Sastre, Adolfo ; Ruchala, Piotr ; Lehrer, Robert I. ; Chang, Theresa ; Klotman, Mary E. / α-defensin inhibits influenza virus replication by cell-mediated mechanism(s). In: Journal of Infectious Diseases. 2007 ; Vol. 196, No. 6. pp. 835-843.
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Salvatore, M, García-Sastre, A, Ruchala, P, Lehrer, RI, Chang, T & Klotman, ME 2007, 'α-defensin inhibits influenza virus replication by cell-mediated mechanism(s)', Journal of Infectious Diseases, vol. 196, no. 6, pp. 835-843. https://doi.org/10.1086/521027

α-defensin inhibits influenza virus replication by cell-mediated mechanism(s). / Salvatore, Mirella; García-Sastre, Adolfo; Ruchala, Piotr; Lehrer, Robert I.; Chang, Theresa; Klotman, Mary E.

In: Journal of Infectious Diseases, Vol. 196, No. 6, 15.09.2007, p. 835-843.

Research output: Contribution to journalArticle

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