β-Sitosterol and stigmasterol ameliorate dextran sulfate sodium-induced colitis in mice fed a high fat Western-style diet

Simin Feng, Zhuqing Dai, Anna Liu, Hong Wang, Jayson Chen, Zisheng Luo, Chung Yang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Phytosterols, the plant analogues of cholesterol, widely occur in the human diet. In this study, we investigated and compared the effects of stigmasterol and β-sitosterol (both with purities ≥95%) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6J male mice fed a high fat Western-style diet. Mice treated with DSS developed severe mucosal colitis, with a marked distortion and crypt loss of colonic surface epithelium. Both β-sitosterol and stigmasterol significantly inhibited colon shortening, lowered fecal hemoglobin content, and reduced the severity of colitis in the middle and distal colon (p < 0.05). These phytosterols also significantly suppressed the activation of nuclear factor-kappa B. They also significantly decreased colony stimulating factor-1 and the nuclear translocation of inflammatory master regulator nuclear factor-kappa B. Stigmasterol significantly lowered the colonic inflammation score and the expression of cyclooxygenase-2 and colony stimulating factor-1, while β-sitosterol was less or not effective. These results suggest that dietary intake of stigmasterol and β-sitosterol ameliorates colitis. Such activities of stigmasterol and β-sitosterol in humans remain to be investigated.

Original languageEnglish (US)
Pages (from-to)4179-4186
Number of pages8
JournalFood and Function
Volume8
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Stigmasterol
Dextran Sulfate
sodium sulfate
sitosterols
stigmasterol
colitis
dextran
Colitis
Fats
Diet
macrophage colony-stimulating factor
mice
lipids
Phytosterols
diet
Macrophage Colony-Stimulating Factor
NF-kappa B
phytosterols
colon
Colon

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

Feng, Simin ; Dai, Zhuqing ; Liu, Anna ; Wang, Hong ; Chen, Jayson ; Luo, Zisheng ; Yang, Chung. / β-Sitosterol and stigmasterol ameliorate dextran sulfate sodium-induced colitis in mice fed a high fat Western-style diet. In: Food and Function. 2017 ; Vol. 8, No. 11. pp. 4179-4186.
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β-Sitosterol and stigmasterol ameliorate dextran sulfate sodium-induced colitis in mice fed a high fat Western-style diet. / Feng, Simin; Dai, Zhuqing; Liu, Anna; Wang, Hong; Chen, Jayson; Luo, Zisheng; Yang, Chung.

In: Food and Function, Vol. 8, No. 11, 01.11.2017, p. 4179-4186.

Research output: Contribution to journalArticle

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