[14C]Aflatoxin B1 as an Indicator of Toxin Destruction during Ammoniation of Contaminated Peanut Meal

Louise S. Lee, Edith J. Conkerton, Robert L. Ory, Joan Bennett

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

[14C]Aflatoxin B, was used to trace the destruction of aflatoxin during ammoniation. Spiked meal(meal containing added [14C]aflatoxin) was ammoniated at 75 °C, 35 psig, for 30 min in alaboratory reactor, and the distribution of label was measured in subsequent fractions. Only 45-50% of the activity was detected in the ammoniated meal after initial air-drying, 8% was detected in the humins remaining after acid hydrolysis, 4-6% was associated with the protein, and 33-36% was associated with the nonprotein residue. Thisresidue contained all of the unreacted aflatoxin B1 which accounted for 0.3% of the total activity. Some activity was detected in volatiles swept from the reaction vessel after the ammoniation treatment.

Original languageEnglish (US)
Pages (from-to)598-602
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume27
Issue number3
DOIs
StatePublished - Feb 1 1979
Externally publishedYes

Fingerprint

peanut meal
Aflatoxin B1
Aflatoxins
aflatoxin B1
aflatoxins
Meals
toxins
humin
Labels
Hydrolysis
Drying
air drying
acid hydrolysis
Acids
Air
Proteins
Arachis
proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "[14C]Aflatoxin B, was used to trace the destruction of aflatoxin during ammoniation. Spiked meal(meal containing added [14C]aflatoxin) was ammoniated at 75 °C, 35 psig, for 30 min in alaboratory reactor, and the distribution of label was measured in subsequent fractions. Only 45-50{\%} of the activity was detected in the ammoniated meal after initial air-drying, 8{\%} was detected in the humins remaining after acid hydrolysis, 4-6{\%} was associated with the protein, and 33-36{\%} was associated with the nonprotein residue. Thisresidue contained all of the unreacted aflatoxin B1 which accounted for 0.3{\%} of the total activity. Some activity was detected in volatiles swept from the reaction vessel after the ammoniation treatment.",
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[14C]Aflatoxin B1 as an Indicator of Toxin Destruction during Ammoniation of Contaminated Peanut Meal. / Lee, Louise S.; Conkerton, Edith J.; Ory, Robert L.; Bennett, Joan.

In: Journal of Agricultural and Food Chemistry, Vol. 27, No. 3, 01.02.1979, p. 598-602.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Lee, Louise S.

AU - Conkerton, Edith J.

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AU - Bennett, Joan

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