[14C]Aflatoxin B, was used to trace the destruction of aflatoxin during ammoniation. Spiked meal(meal containing added [14C]aflatoxin) was ammoniated at 75 °C, 35 psig, for 30 min in alaboratory reactor, and the distribution of label was measured in subsequent fractions. Only 45-50% of the activity was detected in the ammoniated meal after initial air-drying, 8% was detected in the humins remaining after acid hydrolysis, 4-6% was associated with the protein, and 33-36% was associated with the nonprotein residue. Thisresidue contained all of the unreacted aflatoxin B1 which accounted for 0.3% of the total activity. Some activity was detected in volatiles swept from the reaction vessel after the ammoniation treatment.
All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)