31P-Nuclear Magnetic Resonance Studies of the Effect of Recombinant Human Interleukin la on the Bioenergetics of RIF-1 Tumors

Ioannis Constantinidis, Janna P. Wehrle, Jerry D. Glickson, Paul G. Braunschweiger, Nirmal Kumar, Candace S. Johnson, Philip Furmanski

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36 Scopus citations

Abstract

The effect of a single injection of human recombinant interleukin la (IL-1α) on S.C. RIF-1 tumors in mice was studied by in vivo 31,P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-la. At 2, 4, 6, and 8 h after IL-la injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P1 to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P1 and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-la treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-la treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-lα. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.

Original languageEnglish (US)
Pages (from-to)6379-6382
Number of pages4
JournalCancer Research
Volume49
Issue number22
StatePublished - Nov 15 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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