The effect of a single injection of human recombinant interleukin la (IL-1α) on S.C. RIF-1 tumors in mice was studied by in vivo 31,P nuclear magnetic resonance spectroscopy. Spectra were obtained before and up to 24 h after IL-la. At 2, 4, 6, and 8 h after IL-la injection, RIF-1 tumors exhibited a reduction in bioenergetic status compared to untreated controls. The P1 to β-nucleoside triphosphate and the phosphomonoester to β-nucleoside triphosphate ratios increased, while the phosphocreatine to P1 and phosphodiester to phosphomonoester ratios decreased. Tumor blood flow, estimated by 86RbCl uptake, decreased within 30 min after IL-la treatment. Minimum perfusion was detected at 4 h, with recovery between 6 and 12 h after IL-la treatment. Histological sections of the RIF-1 tumors revealed intravascular congestion by 2 h, extravascular hemorrhage by 4 h, and necrosis by 12 h after treatment with IL-lα. The time course of bioenergetic changes in RIF-1 tumors determined by 31P-NMR spectroscopy was found to parallel the reduction and subsequent recovery of tumor blood flow.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Nov 15 1989|
All Science Journal Classification (ASJC) codes
- Cancer Research