A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement

Bing Liu, Andrey Shadrin, Carol Sheppard, Vladimir Mekler, Yingqi Xu, Konstantin Severinov, Steve Matthews, Sivaramesh Wigneshweraraj

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development the multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic b and b' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the p70-factor on initial engagement of RNAp with promoter DNA the new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.

Original languageEnglish (US)
Pages (from-to)4294-4305
Number of pages12
JournalNucleic acids research
Volume42
Issue number7
DOIs
StatePublished - Apr 2014

All Science Journal Classification (ASJC) codes

  • Genetics

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