A calorimetric investigation of single stranded base stacking in the ribo-oligonucleotide A7

Kenneth J. Breslauer, Julian M. Sturtevant

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Differential scanning calorimetry has been employed to determine the energy change associated with single stranded base stacking in the ribo-oligonucleotide A7. A total enthaipy change of 20.3 kcal (mole of heptamer)-1 was measured. This corresponds to 2.9 kcal (mole of adenine)-1 or 3.4 kcal (mole of A-A stack)-1 if one assumes that all six stacking interactions are energetically equivalent. These results represent the first direct determination of this important parameter for a ribo-oligonucleotide. It is noted that the calorimetrically determined value reported here is considerably lower than any of the previously published van 't Hoff enthalpies but is consistent with values that can be derived from other calorimetric data.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalBiophysical chemistry
Volume7
Issue number3
DOIs
StatePublished - Nov 1977

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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