A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin

Philip J. Dix; Ferenc Joó; Pál Maliga

doi:10.1007/BF00268664

A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin

Philip J. Dix, Ferenc Joó, Pál Maliga

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Abstract

Cell lines resistant to 50 μg ml^-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis. Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells. KR103 is defective in morphogenesis and plastid development.

Original language	English (US)
Pages (from-to)	285-290
Number of pages	6
Journal	Mgg Molecular & General Genetics
Volume	157
Issue number	3
DOIs	https://doi.org/10.1007/BF00268664
State	Published - Jan 1977
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1007/BF00268664

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title = "A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin",

abstract = "Cell lines resistant to 50 μg ml-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis. Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells. KR103 is defective in morphogenesis and plastid development.",

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year = "1977",

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AU - Joó, Ferenc

AU - Maliga, Pál

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N2 - Cell lines resistant to 50 μg ml-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis. Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells. KR103 is defective in morphogenesis and plastid development.

AB - Cell lines resistant to 50 μg ml-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis. Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells. KR103 is defective in morphogenesis and plastid development.

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A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin

Abstract

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